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Many contemporary social and environmental problems are increasingly ‘wicked.’ Convergence research offers an effective approach to tackle wicked problems by integrating diverse epistemologies, methodologies, and expertise. Yet, there exists little discussion of how to develop and employ a convergence research approach. This article describes our collaborative research efforts to achieve convergence research and team science. For over a decade, we have sought to understand how drug trafficking activities, and the counternarcotics efforts designed to thwart them, catalyze catastrophic changes in landscapes and communities. We first discuss how understanding our wicked problem called for epistemological convergence of diverse data through a team science approach. We then unpack the potential insights and challenges of methodological convergence by drawing upon examples from our land cover and land use change analysis. Third, we argue that the nature of complex, pressing problems requires convergence research to be politically engaged and accountable to the multiple communities affected. This article aims to provide research teams insight into how to pursue epistemological and methodological convergence while attending to the inherent politics of producing knowledge about wicked problems. 

Small GTP-binding proteins represent a highly conserved signaling module in eukaryotes that regulates diverse cellular processes such as signal transduction, cytoskeletal organization and cell polarity, cell proliferation and differentiation, intracellular membrane trafficking and transport vesicle formation, and nucleocytoplasmic transport. These proteins function as molecular switches that cycle between active and inactive states, and this cycle is linked to GTP binding and hydrolysis. In this review, the roles of the plant complement of small GTP-binding proteins in these cellular processes are described, as well as accessory proteins that control their activity, and current understanding of the functions of individual members of these families in plants—with a focus on the model organism Arabidopsis—is presented. Some potential novel roles of these GTPases in plants, relative to their established roles in yeast and/or animal systems, are also discussed. 

Abstract In plants, root hairs undergo a highly polarized form of cell expansion called tip-growth, in which cell wall deposition is restricted to the root hair apex. In order to identify essential cellular components that might have been missed in earlier genetic screens, we identified conditional temperature-sensitive (ts) root hair mutants by ethyl methanesulfonate mutagenesis in Arabidopsis thaliana. Here, we describe one of these mutants, feronia-temperature sensitive (fer-ts). Mutant fer-ts seedlings were unaffected at normal temperatures (20°C), but failed to form root hairs at elevated temperatures (30°C). Map based-cloning and whole-genome sequencing revealed that fer-ts resulted from a G41S substitution in the extracellular domain of FERONIA (FER). A functional fluorescent fusion of FER containing the fer-ts mutation localized to plasma membranes, but was subject to enhanced protein turnover at elevated temperatures. While tip-growth was rapidly inhibited by addition of rapid alkalinization factor 1 (RALF1) peptides in both wild-type and fer-ts mutants at normal temperatures, root elongation of fer-ts seedlings was resistant to added RALF1 peptide at elevated temperatures. Additionally, at elevated temperatures fer-ts seedlings displayed altered reactive oxygen species (ROS) accumulation upon auxin treatment and phenocopied constitutive fer mutant responses to a variety of plant hormone treatments. Molecular modeling and sequence comparison with other Catharanthus roseus receptor-like kinase 1L (CrRLK1L) receptor family members revealed that the mutated glycine in fer-ts is highly conserved, but is not located within the recently characterized RALF23 and LORELI-LIKE-GLYCOPROTEIN 2 binding domains, perhaps suggesting that fer-ts phenotypes may not be directly due to loss of binding to RALF1 peptides. 

Abstract The intensity of deep convective storms is driven in part by the strength of their updrafts and cold pools. In spite of the importance of these storm features, they can be poorly represented within numerical models. This has been attributed to model parameterizations, grid resolution, and the lack of appropriate observations with which to evaluate such simulations. The overarching goal of the Colorado State University Convective CLoud Outflows and UpDrafts Experiment (C 3 LOUD-Ex) was to enhance our understanding of deep convective storm processes and their representation within numerical models. To address this goal, a field campaign was conducted during July 2016 and May–June 2017 over northeastern Colorado, southeastern Wyoming, and southwestern Nebraska. Pivotal to the experiment was a novel “Flying Curtain” strategy designed around simultaneously employing a fleet of uncrewed aerial systems (UAS; or drones), high-frequency radiosonde launches, and surface observations to obtain detailed measurements of the spatial and temporal heterogeneities of cold pools. Updraft velocities were observed using targeted radiosondes and radars. Extensive datasets were successfully collected for 16 cold pool–focused and seven updraft-focused case studies. The updraft characteristics for all seven supercell updraft cases are compared and provide a useful database for model evaluation. An overview of the 16 cold pools’ characteristics is presented, and an in-depth analysis of one of the cold pool cases suggests that spatial variations in cold pool properties occur on spatial scales from O (100) m through to O (1) km. Processes responsible for the cold pool observations are explored and support recent high-resolution modeling results. 

Counterdrug interdiction efforts designed to seize or disrupt cocaine shipments between South American source zones and US markets remain a core US “supply side” drug policy and national security strategy. However, despite a long history of US-led interdiction efforts in the Western Hemisphere, cocaine movements to the United States through Central America, or “narco-trafficking,” continue to rise. Here, we developed a spatially explicit agent-based model (ABM), called “NarcoLogic,” of narco-trafficker operational decision making in response to interdiction forces to investigate the root causes of interdiction ineffectiveness across space and time. The central premise tested was that spatial proliferation and resiliency of narco-trafficking are not a consequence of ineffective interdiction, but rather part and natural consequence of interdiction itself. Model development relied on multiple theoretical perspectives, empirical studies, media reports, and the authors’ own years of field research in the region. Parameterization and validation used the best available, authoritative data source for illicit cocaine flows. Despite inherently biased, unreliable, and/or incomplete data of a clandestine phenomenon, the model compellingly reproduced the “cat-and-mouse” dynamic between narco-traffickers and interdiction forces others have qualitatively described. The model produced qualitatively accurate and quantitatively realistic spatial and temporal patterns of cocaine trafficking in response to interdiction events. The NarcoLogic model offers a much-needed, evidence-based tool for the robust assessment of different drug policy scenarios, and their likely impact on trafficker behavior and the many collateral damages associated with the militarized war on drugs. 

Abstract A multi-agency succession of field campaigns was conducted in southeastern Texas during July 2021 through October 2022 to study the complex interactions of aerosols, clouds and air pollution in the coastal urban environment. As part of the Tracking Aerosol Convection interactions Experiment (TRACER), the TRACER- Air Quality (TAQ) campaign the Experiment of Sea Breeze Convection, Aerosols, Precipitation and Environment (ESCAPE) and the Convective Cloud Urban Boundary Layer Experiment (CUBE), a combination of ground-based supersites and mobile laboratories, shipborne measurements and aircraft-based instrumentation were deployed. These diverse platforms collected high-resolution data to characterize the aerosol microphysics and chemistry, cloud and precipitation micro- and macro-physical properties, environmental thermodynamics and air quality-relevant constituents that are being used in follow-on analysis and modeling activities. We present the overall deployment setups, a summary of the campaign conditions and a sampling of early research results related to: (a) aerosol precursors in the urban environment, (b) influences of local meteorology on air pollution, (c) detailed observations of the sea breeze circulation, (d) retrieved supersaturation in convective updrafts, (e) characterizing the convective updraft lifecycle, (f) variability in lightning characteristics of convective storms and (g) urban influences on surface energy fluxes. The work concludes with discussion of future research activities highlighted by the TRACER model-intercomparison project to explore the representation of aerosol-convective interactions in high-resolution simulations.